Continuous production of Clostridium tetani toxin.
نویسندگان
چکیده
The continuous production of Clostridium tetani toxin has been carried out in a 1-liter stirred culture vessel for as long as 65 days. Toxin production of approximately 120 flocculating units per ml was maintained with a dilution rate of 0.125 hr(-1), a temperature of 34 C, a pH of 7.4, and the addition to the medium of 0.1 g of potassium chloride per liter. The average minimal lethal intraperitoneal dose of the toxin in mice was approximately 10(6) per ml.
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ورودعنوان ژورنال:
- Applied microbiology
دوره 16 1 شماره
صفحات -
تاریخ انتشار 1968